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u-shaped microdialysis probe spectra/por rc hollow fibers  (Spectrum Medical Inc)

 
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    Spectrum Medical Inc u-shaped microdialysis probe spectra/por rc hollow fibers
    Ruthenium red inhibited vasopressin neuron activity in non-pregnant, late-pregnant, and lactating rats. ( A – C ) Ratemeter recordings (in 30 s bins) of the firing rates of vasopressin neurons in non-pregnant ( A ), late-pregnant ( B ), and lactating ( C ) rats before and during <t>microdialysis</t> administration of ruthenium red (RuR; 10 mM). ( D ) Mean firing rate (in 10 min bins ± SEM) of vasopressin neurons in non-pregnant, late-pregnant, and lactating rats before and during microdialysis administration of ruthenium red (10 mM). Ruthenium red induced a similar reduction in the firing rate of vasopressin neurons in non-pregnant, late-pregnant, and lactating rats (REPRODUCTIVE STATUS: F 2,28 = 0.69, p = 0.51; TIME: F 6,28 = 5.94, p < 0.001; interaction between REPRODUCTIVE STATUS and TIME: F 12,32 = 0.29, p = 0.99, two-way repeated measures ANOVA). * p < 0.05 and ** p < 0.01 compared to pre-RuR within TIME, Holm–Sidak post hoc tests. ( E – G ) Mean (±SEM) peak early hazard ( E ), late hazard ( F ), and peak early/mean late hazard ratio ( G ) in vasopressin neurons from non-pregnant, late-pregnant, and lactating rats. There was no effect of REPRODUCTUVE STATUS or TIME on the peak early hazard (F 1,28 = 1.70, p = 0.20), mean late hazard (F 1,26 = 0.37, p = 0.85), or hazard ratio (F 1,26 = 0.21, p = 0.81) of vasopressin neurons during ruthenium red administration, and no interaction between REPRODUCTUVE STATUS and TIME. ( H ). Mean (±SEM) plasma osmolality in non-pregnant, late-pregnant, and lactating rats. Plasma osmolality was affected by reproductive status ( H = 20.0, p < 0.001). * p < 0.05 and ** p < 0.01. Dunn’s post hoc tests compared to non-pregnant rats.
    U Shaped Microdialysis Probe Spectra/Por Rc Hollow Fibers, supplied by Spectrum Medical Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Plasticity in Intrinsic Excitability of Hypothalamic Magnocellular Neurosecretory Neurons in Late-Pregnant and Lactating Rats"

    Article Title: Plasticity in Intrinsic Excitability of Hypothalamic Magnocellular Neurosecretory Neurons in Late-Pregnant and Lactating Rats

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22137140

    Ruthenium red inhibited vasopressin neuron activity in non-pregnant, late-pregnant, and lactating rats. ( A – C ) Ratemeter recordings (in 30 s bins) of the firing rates of vasopressin neurons in non-pregnant ( A ), late-pregnant ( B ), and lactating ( C ) rats before and during microdialysis administration of ruthenium red (RuR; 10 mM). ( D ) Mean firing rate (in 10 min bins ± SEM) of vasopressin neurons in non-pregnant, late-pregnant, and lactating rats before and during microdialysis administration of ruthenium red (10 mM). Ruthenium red induced a similar reduction in the firing rate of vasopressin neurons in non-pregnant, late-pregnant, and lactating rats (REPRODUCTIVE STATUS: F 2,28 = 0.69, p = 0.51; TIME: F 6,28 = 5.94, p < 0.001; interaction between REPRODUCTIVE STATUS and TIME: F 12,32 = 0.29, p = 0.99, two-way repeated measures ANOVA). * p < 0.05 and ** p < 0.01 compared to pre-RuR within TIME, Holm–Sidak post hoc tests. ( E – G ) Mean (±SEM) peak early hazard ( E ), late hazard ( F ), and peak early/mean late hazard ratio ( G ) in vasopressin neurons from non-pregnant, late-pregnant, and lactating rats. There was no effect of REPRODUCTUVE STATUS or TIME on the peak early hazard (F 1,28 = 1.70, p = 0.20), mean late hazard (F 1,26 = 0.37, p = 0.85), or hazard ratio (F 1,26 = 0.21, p = 0.81) of vasopressin neurons during ruthenium red administration, and no interaction between REPRODUCTUVE STATUS and TIME. ( H ). Mean (±SEM) plasma osmolality in non-pregnant, late-pregnant, and lactating rats. Plasma osmolality was affected by reproductive status ( H = 20.0, p < 0.001). * p < 0.05 and ** p < 0.01. Dunn’s post hoc tests compared to non-pregnant rats.
    Figure Legend Snippet: Ruthenium red inhibited vasopressin neuron activity in non-pregnant, late-pregnant, and lactating rats. ( A – C ) Ratemeter recordings (in 30 s bins) of the firing rates of vasopressin neurons in non-pregnant ( A ), late-pregnant ( B ), and lactating ( C ) rats before and during microdialysis administration of ruthenium red (RuR; 10 mM). ( D ) Mean firing rate (in 10 min bins ± SEM) of vasopressin neurons in non-pregnant, late-pregnant, and lactating rats before and during microdialysis administration of ruthenium red (10 mM). Ruthenium red induced a similar reduction in the firing rate of vasopressin neurons in non-pregnant, late-pregnant, and lactating rats (REPRODUCTIVE STATUS: F 2,28 = 0.69, p = 0.51; TIME: F 6,28 = 5.94, p < 0.001; interaction between REPRODUCTIVE STATUS and TIME: F 12,32 = 0.29, p = 0.99, two-way repeated measures ANOVA). * p < 0.05 and ** p < 0.01 compared to pre-RuR within TIME, Holm–Sidak post hoc tests. ( E – G ) Mean (±SEM) peak early hazard ( E ), late hazard ( F ), and peak early/mean late hazard ratio ( G ) in vasopressin neurons from non-pregnant, late-pregnant, and lactating rats. There was no effect of REPRODUCTUVE STATUS or TIME on the peak early hazard (F 1,28 = 1.70, p = 0.20), mean late hazard (F 1,26 = 0.37, p = 0.85), or hazard ratio (F 1,26 = 0.21, p = 0.81) of vasopressin neurons during ruthenium red administration, and no interaction between REPRODUCTUVE STATUS and TIME. ( H ). Mean (±SEM) plasma osmolality in non-pregnant, late-pregnant, and lactating rats. Plasma osmolality was affected by reproductive status ( H = 20.0, p < 0.001). * p < 0.05 and ** p < 0.01. Dunn’s post hoc tests compared to non-pregnant rats.

    Techniques Used: Activity Assay, Clinical Proteomics

    Ruthenium red did not affect oxytocin neuron activity in non-pregnant and late-pregnant rats. ( A , B ) Ratemeter recordings (in 30 s bins) of the firing rates of oxytocin neurons in non-pregnant ( A ) and late-pregnant ( B ) rats before and during microdialysis administration of ruthenium red (RuR; 10 mM). ( C ) Mean firing rate (in 10 min bins ± SEM) of oxytocin neurons in non-pregnant and late-pregnant rats before and during microdialysis administration of ruthenium red (10 mM). Oxytocin neuron activity was not affected by ruthenium red in non-pregnant or late-pregnant rats (REPRODUCTIVE STATUS: F 1,9 = 0.03, p = 0.87; TIME: F 6,9 = 1.10, p = 0.37; interaction between REPRODUCTIVE STATUS and TIME: F 6,9 = 1.00, p = 0.41, two-way repeated measures ANOVA). ( D – F ) Mean (±SEM) peak early hazard ( D ), late hazard ( E ), and peak early/mean late hazard ratio ( F ) in oxytocin neurons from non-pregnant, late-pregnant, and lactating rats. There was no effect of REPRODUCTIVE STATUS or TIME on the peak early hazard (F 1,9 = 0.58, p = 0.46), mean late hazard (F 1,9 = 1.97, p = 0.19), or hazard ratio (F 1,9 = 0.09, p = 0.77) of oxytocin neurons during ruthenium red administration, and no interaction between REPRODUCTUVE STATUS and TIME.
    Figure Legend Snippet: Ruthenium red did not affect oxytocin neuron activity in non-pregnant and late-pregnant rats. ( A , B ) Ratemeter recordings (in 30 s bins) of the firing rates of oxytocin neurons in non-pregnant ( A ) and late-pregnant ( B ) rats before and during microdialysis administration of ruthenium red (RuR; 10 mM). ( C ) Mean firing rate (in 10 min bins ± SEM) of oxytocin neurons in non-pregnant and late-pregnant rats before and during microdialysis administration of ruthenium red (10 mM). Oxytocin neuron activity was not affected by ruthenium red in non-pregnant or late-pregnant rats (REPRODUCTIVE STATUS: F 1,9 = 0.03, p = 0.87; TIME: F 6,9 = 1.10, p = 0.37; interaction between REPRODUCTIVE STATUS and TIME: F 6,9 = 1.00, p = 0.41, two-way repeated measures ANOVA). ( D – F ) Mean (±SEM) peak early hazard ( D ), late hazard ( E ), and peak early/mean late hazard ratio ( F ) in oxytocin neurons from non-pregnant, late-pregnant, and lactating rats. There was no effect of REPRODUCTIVE STATUS or TIME on the peak early hazard (F 1,9 = 0.58, p = 0.46), mean late hazard (F 1,9 = 1.97, p = 0.19), or hazard ratio (F 1,9 = 0.09, p = 0.77) of oxytocin neurons during ruthenium red administration, and no interaction between REPRODUCTUVE STATUS and TIME.

    Techniques Used: Activity Assay



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    Ruthenium red inhibited vasopressin neuron activity in non-pregnant, late-pregnant, and lactating rats. ( A – C ) Ratemeter recordings (in 30 s bins) of the firing rates of vasopressin neurons in non-pregnant ( A ), late-pregnant ( B ), and lactating ( C ) rats before and during microdialysis administration of ruthenium red (RuR; 10 mM). ( D ) Mean firing rate (in 10 min bins ± SEM) of vasopressin neurons in non-pregnant, late-pregnant, and lactating rats before and during microdialysis administration of ruthenium red (10 mM). Ruthenium red induced a similar reduction in the firing rate of vasopressin neurons in non-pregnant, late-pregnant, and lactating rats (REPRODUCTIVE STATUS: F 2,28 = 0.69, p = 0.51; TIME: F 6,28 = 5.94, p < 0.001; interaction between REPRODUCTIVE STATUS and TIME: F 12,32 = 0.29, p = 0.99, two-way repeated measures ANOVA). * p < 0.05 and ** p < 0.01 compared to pre-RuR within TIME, Holm–Sidak post hoc tests. ( E – G ) Mean (±SEM) peak early hazard ( E ), late hazard ( F ), and peak early/mean late hazard ratio ( G ) in vasopressin neurons from non-pregnant, late-pregnant, and lactating rats. There was no effect of REPRODUCTUVE STATUS or TIME on the peak early hazard (F 1,28 = 1.70, p = 0.20), mean late hazard (F 1,26 = 0.37, p = 0.85), or hazard ratio (F 1,26 = 0.21, p = 0.81) of vasopressin neurons during ruthenium red administration, and no interaction between REPRODUCTUVE STATUS and TIME. ( H ). Mean (±SEM) plasma osmolality in non-pregnant, late-pregnant, and lactating rats. Plasma osmolality was affected by reproductive status ( H = 20.0, p < 0.001). * p < 0.05 and ** p < 0.01. Dunn’s post hoc tests compared to non-pregnant rats.

    Journal: International Journal of Molecular Sciences

    Article Title: Plasticity in Intrinsic Excitability of Hypothalamic Magnocellular Neurosecretory Neurons in Late-Pregnant and Lactating Rats

    doi: 10.3390/ijms22137140

    Figure Lengend Snippet: Ruthenium red inhibited vasopressin neuron activity in non-pregnant, late-pregnant, and lactating rats. ( A – C ) Ratemeter recordings (in 30 s bins) of the firing rates of vasopressin neurons in non-pregnant ( A ), late-pregnant ( B ), and lactating ( C ) rats before and during microdialysis administration of ruthenium red (RuR; 10 mM). ( D ) Mean firing rate (in 10 min bins ± SEM) of vasopressin neurons in non-pregnant, late-pregnant, and lactating rats before and during microdialysis administration of ruthenium red (10 mM). Ruthenium red induced a similar reduction in the firing rate of vasopressin neurons in non-pregnant, late-pregnant, and lactating rats (REPRODUCTIVE STATUS: F 2,28 = 0.69, p = 0.51; TIME: F 6,28 = 5.94, p < 0.001; interaction between REPRODUCTIVE STATUS and TIME: F 12,32 = 0.29, p = 0.99, two-way repeated measures ANOVA). * p < 0.05 and ** p < 0.01 compared to pre-RuR within TIME, Holm–Sidak post hoc tests. ( E – G ) Mean (±SEM) peak early hazard ( E ), late hazard ( F ), and peak early/mean late hazard ratio ( G ) in vasopressin neurons from non-pregnant, late-pregnant, and lactating rats. There was no effect of REPRODUCTUVE STATUS or TIME on the peak early hazard (F 1,28 = 1.70, p = 0.20), mean late hazard (F 1,26 = 0.37, p = 0.85), or hazard ratio (F 1,26 = 0.21, p = 0.81) of vasopressin neurons during ruthenium red administration, and no interaction between REPRODUCTUVE STATUS and TIME. ( H ). Mean (±SEM) plasma osmolality in non-pregnant, late-pregnant, and lactating rats. Plasma osmolality was affected by reproductive status ( H = 20.0, p < 0.001). * p < 0.05 and ** p < 0.01. Dunn’s post hoc tests compared to non-pregnant rats.

    Article Snippet: Following the removal of the meninges overlying the supraoptic nucleus, a U-shaped microdialysis probe [ ], permeable to 10 kDa (Spectra/Por RC Hollow Fibers, Spectrum Medical Inc., Houston, TX, USA), was bent to position the loop of the membrane over the ventral surface of the supraoptic nucleus.

    Techniques: Activity Assay, Clinical Proteomics

    Ruthenium red did not affect oxytocin neuron activity in non-pregnant and late-pregnant rats. ( A , B ) Ratemeter recordings (in 30 s bins) of the firing rates of oxytocin neurons in non-pregnant ( A ) and late-pregnant ( B ) rats before and during microdialysis administration of ruthenium red (RuR; 10 mM). ( C ) Mean firing rate (in 10 min bins ± SEM) of oxytocin neurons in non-pregnant and late-pregnant rats before and during microdialysis administration of ruthenium red (10 mM). Oxytocin neuron activity was not affected by ruthenium red in non-pregnant or late-pregnant rats (REPRODUCTIVE STATUS: F 1,9 = 0.03, p = 0.87; TIME: F 6,9 = 1.10, p = 0.37; interaction between REPRODUCTIVE STATUS and TIME: F 6,9 = 1.00, p = 0.41, two-way repeated measures ANOVA). ( D – F ) Mean (±SEM) peak early hazard ( D ), late hazard ( E ), and peak early/mean late hazard ratio ( F ) in oxytocin neurons from non-pregnant, late-pregnant, and lactating rats. There was no effect of REPRODUCTIVE STATUS or TIME on the peak early hazard (F 1,9 = 0.58, p = 0.46), mean late hazard (F 1,9 = 1.97, p = 0.19), or hazard ratio (F 1,9 = 0.09, p = 0.77) of oxytocin neurons during ruthenium red administration, and no interaction between REPRODUCTUVE STATUS and TIME.

    Journal: International Journal of Molecular Sciences

    Article Title: Plasticity in Intrinsic Excitability of Hypothalamic Magnocellular Neurosecretory Neurons in Late-Pregnant and Lactating Rats

    doi: 10.3390/ijms22137140

    Figure Lengend Snippet: Ruthenium red did not affect oxytocin neuron activity in non-pregnant and late-pregnant rats. ( A , B ) Ratemeter recordings (in 30 s bins) of the firing rates of oxytocin neurons in non-pregnant ( A ) and late-pregnant ( B ) rats before and during microdialysis administration of ruthenium red (RuR; 10 mM). ( C ) Mean firing rate (in 10 min bins ± SEM) of oxytocin neurons in non-pregnant and late-pregnant rats before and during microdialysis administration of ruthenium red (10 mM). Oxytocin neuron activity was not affected by ruthenium red in non-pregnant or late-pregnant rats (REPRODUCTIVE STATUS: F 1,9 = 0.03, p = 0.87; TIME: F 6,9 = 1.10, p = 0.37; interaction between REPRODUCTIVE STATUS and TIME: F 6,9 = 1.00, p = 0.41, two-way repeated measures ANOVA). ( D – F ) Mean (±SEM) peak early hazard ( D ), late hazard ( E ), and peak early/mean late hazard ratio ( F ) in oxytocin neurons from non-pregnant, late-pregnant, and lactating rats. There was no effect of REPRODUCTIVE STATUS or TIME on the peak early hazard (F 1,9 = 0.58, p = 0.46), mean late hazard (F 1,9 = 1.97, p = 0.19), or hazard ratio (F 1,9 = 0.09, p = 0.77) of oxytocin neurons during ruthenium red administration, and no interaction between REPRODUCTUVE STATUS and TIME.

    Article Snippet: Following the removal of the meninges overlying the supraoptic nucleus, a U-shaped microdialysis probe [ ], permeable to 10 kDa (Spectra/Por RC Hollow Fibers, Spectrum Medical Inc., Houston, TX, USA), was bent to position the loop of the membrane over the ventral surface of the supraoptic nucleus.

    Techniques: Activity Assay